Clinical immunity
Low-frequency CD8⁺ T cells induced by SIGN-R1⁺ macrophage-targeted vaccine confer SARS-CoV-2 clearance in mice

A research group led by Dr. Daisuke MURAOKA, Division of Translational Oncoimmunology, Aichi Cancer Center Research Institute, Dr. MOI MENG LING, School of International Health, Graduate School of Medicine, the University of Tokyo, Dr. Kazunari AKIYOSHI, Department of Immunology, Graduate School of Medicine, Kyoto University, Dr. Hiroaki IKEDA, Department of Oncology, Nagasaki University Graduate School of Biomedical Sciences, and Dr. Naozumi Harada, United Immunity, Co., Ltd.,, have demonstrated that the selective delivery of PNG to medullary macrophages depends on its binding to the C-type lectin SIGN-R1.
This induction of specific CD8-positive killer T cells that respond rapidly to viral infection, even at low frequencies, is critical for vaccine efficacy and can be achieved by targeting SIGN-R1⁺ myeloid macrophages.
In this study, our T-cell receptor (TCR) Repertoire Analysis technology was used as a method to identify clonal differences in induced CD8-positive killer T cells.

The results of this research are published in a news release on the following website.

Tokyo University: Research publication on PNG based infectious disease vaccine development

Kyoto University: 我が国独自のナノ粒子性薬剤送達システムを用いた次世代ワクチンの新型コロナウイルスに対する優れたキラーT細胞誘導と感染防御性能を動物モデルで実証―将来の感染症ワクチン開発への幅広い応用の可能性―　(Japanese)

Nagasaki University: T細胞誘導と感染防御性能を動物モデルで実証　(Japanese)

Aichi Cancer Center: T細胞誘導と感染防御性能を動物モデルで実証　(Japanese)

United Immunity Co., Ltd.: A Next-Generation COVID-19 Vaccine Using Myeloid Targeting PlatformTM Shows Superior Killer T Cell Induction and Infection Protection Properties in a Preclinical Animal Model

Clinical immunity
Analysis of B-cell receptor repertoire to evaluate immunogenicity of monovalent Omicron XBB.1.5 mRNA vaccines

A paper utilizing our Repertoire Analysis was published by Dr. Yohei Funakoshi and Dr. Kimikazu Yakushijin, the Department of Medicine, Division of Medical Oncology/Hematology, Kobe University Hospital and Graduate School of Medicine, and Dr. Goh Ohji, the Department of Microbiology and Infectious Diseases, Division of Infection Disease Therapeutics, Kobe University Hospital.

In this paper, they evaluated the Omicron XBB.1.5 mRNA vaccine using a new novel coronavirus-specific antibody sequence quantification method developed in the laboratory (QASAS method), and the results suggest that antibody production against the Omicron XBB strain is stronger than that of previous vaccines.

Our BCR Repertoire Analysis is used to obtain antibody gene sequences for matching against the novel coronavirus-specific antibody database.

Basal immunity
Human iPSC-derived CD4⁺ Treg-like cells engineered with chimeric antigen receptors control GvHD in a xenograft model

A paper utilizing our TCR Repertoire Analysis was published by Dr. Hisashi Yano, Shin Kaneko Laboratory, CiRA, Kyoto University.
In this paper, they report a success in producing CD4⁺ Treg-like cells derived from human iPS cells by inducing FOXP3 expression.
Using our TCR Repertoire Analysis, it was confirmed that cells cultured from non-T cell-derived iPS cells reconstitute various TCRα and β genes during their differentiation, constituting a polyclonal TCR repertoire.

Chronic ingestion of soy peptide supplementation reduces aggressive behavior and abnormal fear memory caused by juvenile social isolation

A paper utilizing our Bacterial Flora Analysis was published by Dr. Hideki Tamura, et al., Laboratory of Bifunctional Science, School of Pharmacy and Pharmaceutical Sciences, Hoshi University.
In this paper, they investigated the effects of soy peptides on the composition of the gut microbiota have been examined, suggesting that they may provide evidence that the gut microbiota influences brain function via metabolic, endocrine, immune, and neural pathways.
Our 16S rRNA Bacterial Flora Analysis was performed using mouse feces in the paper.

Peripheral-central network analysis of cancer cachexia status accompanied by the polarization of hypothalamic microglia with low expression of inhibitory immune checkpoint receptors

A paper utilizing our Bacterial Flora Analysis was published by Dr. Yukari Suda, Dr. Yusuke Hamada et al., Department of Pharmacology, Hoshi University School of Pharmacy and Pharmaceutical Sciences.
In this paper, they investigated to demonstrate the relationship between the induction of cancer cachexia and its effects on the intestinal microbiota.
And they suggest that the release of LPS from the gut microbiota may exacerbate the inflammatory state of the hypothalamus with polarization toward microglia expressing low levels of inhibitory immune checkpoint receptors.
Our 16S rRNA Bacterial Flora Analysis was performed using mouse feces in the paper.

Hematological malignancy
Decade-long WT1-specific CTLs induced by WT1 peptide vaccination

A paper utilizing our TCR Repertoire Analysis was published by Dr. Tatsuya Suwabe et al., Department of Hematopoietic Stem Cell Transplantation, Niigata University Medical and Dental Hospital.
In this paper, they investigated how long and to what extent WT1-specific CD8+ cytotoxic T cells (CTL) persisted after WT1 peptide vaccination, and reported that the immune response persisted for more than 10 years even after vaccination was discontinued.
Our TCR Repertoire Analysis was performed using WT1-specific CTLs after mixed lymphocyte peptide culture and revealed the diversity of WT1-specific CTLs 11 years after vaccination.